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tslp  (R&D Systems)


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    Structured Review

    R&D Systems tslp
    Tslp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tslp/Human+TSLP+DuoSet+ELISA/10__1016_slash_j__microc__2026__117495-97-64-69
    Average 94 stars, based on 25 article reviews
    tslp - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Neutralization:

    Article Title: Eosinophil extracellular traps drive asthma progression through neuro-immune signals.
    Article Snippet: 1Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.. 2Breast Tumor Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.. 3Department of Pulmonary and Critical Care Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.

    Incubation:

    Article Title: TSLP up‐regulates inflammatory responses through induction of autophagy in T cells
    Article Snippet: 1College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 2Korean MedicineBased Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 3Center for Converging Humanities, Kyung Hee University, Seoul, Republic of Korea 4Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 5Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 6Department of Anatomy & Information Sciences, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 7Department of Food Science & Technology, Hoseo University, Asan, Republic of Korea

    Article Title: JAK2 Disease-Risk Variants are Gain of Function and JAK Signaling Threshold Determines Innate Receptor-Induced Proinflammatory Cytokine Secretion in Macrophages
    Article Snippet: Myeloid cell stimulation Monocyte-derived macrophages (MDMs) were cultured with muramyl dipeptide (MDP) (Bachem, Torrance, CA), recombinant IL-10, IL-4, IL-22 (R&D Systems) or TSLP (Peprotech). .. For inhibitor and neutralizing antibody treatments, cells were incubated with JAK inhibitor I (total JAK inhibitor), SD-1029 (JAK2 inhibitor) (Calbiochem, La Jolla, CA), tofacitinib (manufactured by Pfizer, NY, NY), neutralizing anti-IL-10 (5 μg/ml), anti-IL-4 (1 μg/ml; BD Biosciences, San Jose, CA), anti-IL-22 (1.25 μg/ml; Peprotech) or anti-TSLP (0.25 μg/ml; R&D Systems) antibodies 1h prior to treatment. .. Supernatants were assayed for IL-10 (BD Biosciences), or IL-1β, IL-4, IL-6, IL-8, IL-12, IL-1ra (eBioscience, San Diego, CA), IL-22 (Peprotech) or TSLP (R&D Systems) by ELISA.

    Recombinant:

    Article Title: TSLP up‐regulates inflammatory responses through induction of autophagy in T cells
    Article Snippet: 1College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 2Korean MedicineBased Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 3Center for Converging Humanities, Kyung Hee University, Seoul, Republic of Korea 4Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 5Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 6Department of Anatomy & Information Sciences, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea 7Department of Food Science & Technology, Hoseo University, Asan, Republic of Korea

    Fluorescence:

    Article Title: Interferon-λ enhances adaptive mucosal immunity by boosting release of thymic stromal lymphopoietin.
    Article Snippet: 1Institute of Virology, Medical Center University of Freiburg, Freiburg, Germany.. 2Spemann Graduate School of Biology and Medicine, Albert Ludwigs University Freiburg, Freiburg, Germany.. 3Institute of Medical Microbiology, Medical Center University of Freiburg, Freiburg, Germany.

    Blocking Assay:

    Article Title: Comparative Analysis of Thymic and Blood Treg in Myasthenia Gravis: Thymic Epithelial Cells Contribute to Thymic Immunoregulatory Defects
    Article Snippet: .. In some experiments, blocking anti-TSLP (R&D Systems, Lille, France) antibody was used at 0.1 μg/ml. .. Thymic stromal lymphopoietin (TSLP) concentrations were measured in duplicate from supernatants of cultures of TECs, using commercially available enzyme-linked immunosorbent assays (Peprotech, France) according to the manufacturer’s instructions.

    Modification:

    Article Title: Use of Physcion to Improve Atopic Dermatitis-Like Skin Lesions through Blocking of Thymic Stromal Lymphopoietin
    Article Snippet: .. We purchased physcion (purity ≥ 98%, A) from Cayman Chemical Company (Ann Arbor, MI, USA); Isocove’s Modified Dulbecco’s Medium (IMDM) from Gibco BRL (Grand Island, NY, USA); fetal bovine serum (FBS) from Welgene (Daegu, Korea); DEX, PMACI, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), BAPTA-AM, Fura-2/AM, DNFB, and O -phthaldialdehyde from Sigma Chemical Co. (St. Louis, MO, USA); RPMI Media 1640 from Thermo Fisher Scientific Inc. (Waltham, MA, USA); anti-TSLP from R & D system Inc. (Minneapolis, MN, USA), IL-6 from Pharmingen (Sandiego, CA, USA), TNF-α (Pharmingen), IL-1β (R & D system Inc.), IL-4 (Pharmingen), IFN-γ (Pharmingen), and IgE antibodies (Pharmingen); anti- pERK, ERK, pJNK, JNK, pp38, p38, RIP2, caspase-1, pIKKβ, NF-κB, pIκBα, poly-ADP-ribose polymerase (PARP), GAPDH, and peroxidase-conjugated second antibodies from Santa Cruz Biotechnology (Santa Cruz, CA, USA); RIPA buffer from Cell signaling (Billerica, MA, USA). ..

    MTT Assay:

    Article Title: Use of Physcion to Improve Atopic Dermatitis-Like Skin Lesions through Blocking of Thymic Stromal Lymphopoietin
    Article Snippet: .. We purchased physcion (purity ≥ 98%, A) from Cayman Chemical Company (Ann Arbor, MI, USA); Isocove’s Modified Dulbecco’s Medium (IMDM) from Gibco BRL (Grand Island, NY, USA); fetal bovine serum (FBS) from Welgene (Daegu, Korea); DEX, PMACI, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), BAPTA-AM, Fura-2/AM, DNFB, and O -phthaldialdehyde from Sigma Chemical Co. (St. Louis, MO, USA); RPMI Media 1640 from Thermo Fisher Scientific Inc. (Waltham, MA, USA); anti-TSLP from R & D system Inc. (Minneapolis, MN, USA), IL-6 from Pharmingen (Sandiego, CA, USA), TNF-α (Pharmingen), IL-1β (R & D system Inc.), IL-4 (Pharmingen), IFN-γ (Pharmingen), and IgE antibodies (Pharmingen); anti- pERK, ERK, pJNK, JNK, pp38, p38, RIP2, caspase-1, pIKKβ, NF-κB, pIκBα, poly-ADP-ribose polymerase (PARP), GAPDH, and peroxidase-conjugated second antibodies from Santa Cruz Biotechnology (Santa Cruz, CA, USA); RIPA buffer from Cell signaling (Billerica, MA, USA). ..



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    Image Search Results


    Therapeutic neutralization of TSLP protects against AAA development. (A) Schematic diagram of the experimental protocol for anti-mouse TSLP antibody (anti-TSLP) administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for Elastica Van Gieson (EVG) (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 50 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: Therapeutic neutralization of TSLP protects against AAA development. (A) Schematic diagram of the experimental protocol for anti-mouse TSLP antibody (anti-TSLP) administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for Elastica Van Gieson (EVG) (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 50 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Article Snippet: Anti-TSLP antibody treatment: Anti-TSLP antibody (HY-P990150, MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Neutralization, Comparison, Saline, Staining, Immunohistochemical staining, Expressing, Two Tailed Test, Control